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  • 국내학술대회

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    국내학술대회 제목 게시판 내용
    제목(국문) Evaluation of endothelial cell adhesion for vascular networks under perfusion conditions
    제목(영문) Evaluation of endothelial cell adhesion for vascular networks under perfusion conditions
    저자 강태연 (Kang, Tae-Yun ,POSTECH ) ▷공저자네트워크등록하기
    홍정민 (Hong, Jung-min ,POSTECH ) ▷공저자네트워크등록하기
    정진우 (Jung, Jin-woo ,POSTECH ) ▷공저자네트워크등록하기
    강경신 (Kyung Shin Kang ,POSTECH ) ▷공저자 네트워크 보기
    차형준 (Cha, Hyung-Joon ,POSTECH ) ▷공저자네트워크등록하기
    조동우 (Cho, Dong-woo ,POSTECH ) ▷공저자네트워크등록하기
    초록

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    초록(영문)

    The aim of this study was to evaluate the effects of collagen and mussel adhesive protein (MAP) coating on endothelial cell adhesion under perfusion culture conditions. Three kinds of scaffolds were prepared: (I) PCL scaffold fabricated by salt leaching method, (2) PCL scaffold coated with collagen, (3) PCL scaffold coated with collagen and MAP. Scaffolds were loaded in PDMS chambers which were connected with perfusion system. The magnitude of shear stress acting on the scaffold surface was estimated by FEM analysis. After seeding cells on the scaffold surface, they were cultured for 6 days under perfusion condition. DNA quantification, real-time PCR, SEM imaging and immnostaining were used for evaluating the cell adhesion. The results showed that perfusion culture could cause cell detaclnnent at the initial state, but it promoted gene expressions related with cell adhesion and ECM secretion in the long term. Especially, MAP coating and perfusion condition showed synergetic effects on gene expressions and ECM secretion.

    keyword Scaffold, Endothelial Cell, Perfusion Culture
    저널명 대한기계학회 춘계학술대회 ▷관련저널보기
    VOL 11
    PAGE 0019
    발표년도 2011
    원문파일 국문다운로드
    • 페이스북아이콘
    • 트위터 아이콘

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