Welcome to BMNL (Bioanalytical Microfludidics and Nanofluidics Laboratory)

Our lab is mainly interested in application of microfluidic and nanofluidic technologies to analyze important biomolecules such as proteins, peptide, RNA, and DNA. We are also exploring interesting physochemical phenomena in microfluidic and nanofluidic systems that are not observable in conventional  macro-scale fluidic behaviors. Microfabrication technology, orignially invented in the microelectronics industry, has been successfully empployed to fabricate fluidic components such as channels, pumps, valves, mixer, and chamber in high precision and in a highly integrated fashion as can be seen below:

As a means of "plumbing", we are investigating effective methods of mobilizing biomolecules inside microfluidic device, such as electrophoresis, isotachophoresis , magnetophoresis, and dielectric phoresis. More complicated bioassay such as chromatin immunoprecipitation can be performed on chip by combining each mass transfer modality.  Prior to analysis of biomolecules , the molecules should be strongly immobilized  on microfluidic channel surfaces because the measurable signal is proportional to the amount immobilized (e.g., using electrostatic interaction in the following image). Our lab is also studying molecular interaction between biomolecules and solid substrate as a means of improving detection sensitivity.

Ultimately, we are pursuing microanalytical systems that can determine clinically and biologically important biomolecules accurately, rapidly, and reliably - so that they can replace bulky, expensive, material-consuming, but essential analytical instruments in research or clinical laboratory.

 

Minsub Chung, Dohyun Kim, and Amy E. Herr
Microfluidic Sodium Dodecyl Sulfate Western Blotting
in preparation, , Vol. 0, No. 0, pp. 0~ 0

Dohyun Kim, and Amy E. Herr
Immobilization matrix for microfluidic applications
in preparation, , Vol. 0, No. 0, pp. 0~ 0

Dohyun Kim, and Jack W. Judy
A miniaturized parallel-plate Donnan-dialytic sample-preparation system using anion-exchange membrane
in preparation, , Vol. 0, No. 0, pp. 0~ 0

Dohyun Kim, Kelly Karns, Samuel Q. Tia, Mei He, and Amy E. Herr
Electrostatic Immobilization of Proteins on Charged Polyacrylamide Gels for Cationic Detergent Microfluidic Western Blotting
Analytical Chemistry, 2012-03, Vol. 84, No. 0, pp. 2533~ 2540

Samuel Tia, Mei He, Dohyun Kim, and Amy E. Herr
Multi-analyte On-Chip Native Western Blotting
Analytical Chemistry, 2011-03, Vol. 83, No. 0, pp. 3581~ 3588

Minh Khang Chau, Nebiyu Getachew Arega, Nguyen Anh Nhung Tran, Jin Song, Sangmin Lee, Jintae Kim, Minsub Chung, and Dohyun Kim
"Capacitively Coupled Contactless Conductivity Detection for Microfluidic Capillary Isoelectric Focusing"
Analytical Chimica Acta, 2020-03, Vol. 1125, No. 0, pp. 60~ 70

Yeji Kim, Kyung Joo Song, Jintae Kim, Minsub Chung, and Dohyun Kim
”Single Amino Acid Replacement transforms mCherry to a Far-red fluorescence protein"
Biotechnology and Bioprocess Engineering, 2017-01, Vol. 21, No. 0, pp. 720~ 725

Hakhyun Kim, Heewon Hwang, Seonhyeok Baek, and Dohyun Kim
"Design, Fabrication, and Performance Evaluation of a Printed-circuit-board Microfluidic Electrolytic Pump for Lab-on-a-chip Devices
Sensors & Actuators: A. Physical, 2018-07, Vol. 277, No. 0, pp. 73~ 84